Root Cause Analysis of Invalid Internal Control in COVID-19 RT-PCR Testing: A Learning Experience

COVID-19 in Nursing Homes: The Problematic Management of Residents Without Positive COVID-19 RT-PCR

Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

Comparing the Diagnostic Power of Chest CT Scan and RT-PCR in Diagnosis of COVID-19

Background and purpose: Proper diagnosis of patients with COVID-19 is one of the challenges in medical centers. RT-PCR is the standard and reference test in diagnosis of patients with COVID-19. This study aimed at accelerating the correct diagnosis of COVID-19 and the diagnostic power of chest CT scan and RT-PCR. Materials and methods: This study was performed in 569 patients with COVID-19 adm...

Internal control for quality assurance of diagnostic RT-PCR.

Reverse transcription polymerase chain reaction (RT-PCR) is gaining major importance in the field of medical diagnostics. For example, RT-PCR assays are being tested to become standard methodology for detection of occult tumor cells in peripheral blood of cancer patients. However, there is heterogeneity in the frequency of tumor cell detection, e.g., in melanoma patients as reported in the lite...

Quantitative RT-PCR using a PCR-generated competitive internal standard.

One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...